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primary anti bodies  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary anti bodies
    Primary Anti Bodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+anti+bodies/pmc12447342__mmc4-231-0-17
    Average 86 stars, based on 1 article reviews
    primary anti bodies - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Loss of genome maintenance is linked to mTORC1 signaling and accelerates podocyte damage.
    Article Snippet: .. Primary anti-bodies (anti-gH2A.X #2577s – Cell Signaling Technology, anti-nephrin 498 #GP-N2 – Progen, anti-synaptopodin #65294 – Progen, anti-Dach1 #HPA012672 – Sigma Aldrich 499 (75), anti-phospho-S6 Ribosomal Protein (Ser235/236) #4858 – Cell Signaling Technology, anti-500 phospho-STING (Ser336) # 19781 – Cell Signaling Technology, anti-phospho-PKC alpha 501 (Ser657) # sc-12356 – Santa Cruz Biotechnology and anti-p53 #p53-protein-cm5 Leica 502 Biosystems, anti-ATM (phospho S1987) antibody [EPR28058-71] # ab315019 – abcam, anti-DNA 503 PKcs (phospho S2056) antibody - ChIP Grade # ab18192 – abcam) were used at 1:200 dilution. ..

    Incubation:

    Article Title: Ribosomal protein L5 mediated inhibition of c-Myc is critically involved in sanggenon G induced apoptosis in non-small lung cancer cells.
    Article Snippet: Funding information National Research Foundation of Korea, Grant/Award Number: 2020R1A5A201941311; Rural Development Administration, Grant/Award Number: PJ01317002 Though Sanggenon G (SanG) from root bark of Morus alba was known to exhibit antioxidant and anti-depressant effects, its underlying mechanisms still remain unclear.. Herein SanG reduced the viability of A549 and H1299 non-small lung cancer cells (NSCLCs).. Also, SanG increased sub-G1 population via inhibition of cyclin D1, cyclin E, CDK2, CDK4 and Bcl-2, cleavages of poly (ADP-ribose) polymerase (PARP) and caspase-3 in A549 and H1299 cells.

    Article Title: N-acetylcysteine and Hydroxychloroquine Ameliorate ADMA-Induced Fetal Growth Restriction in Mice via Regulating Oxidative Stress and Autophagy.
    Article Snippet: Fetal growth restriction (FGR) seriously threatens perinatal health.. The main cause of FGR is placental malperfusion, but the specific mechanism is still unclear, and there is no effective treatment for FGR.. We constructed a FGR mouse model by adding exogenous asymmetric dimethylarginine (ADMA) through in vivo experiments and found that ADMA could cause placental dysplasia and induce the occurrence of FGR.

    Cell-Signaling:

    Article Title: Pilose antler aqueous extract promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells by stimulating the BMP-2/Smad1, 5/Runx2 signaling pathway
    Article Snippet: [ABSTRACT] Peptides from Pilose antler aqueous extract (PAAE) have been shown to stimulate the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs).. However, the underlying molecular mechanisms are not well understood.. Here, PAAE was isolated and purified to explore the molecular mechanisms underlying PAAE’s effects on BMSCs as well as its osteoprotective effects in ovariectomized rats.

    Activity Assay:

    Article Title: The age-dependent development of abnormal cardiac metabolism in the peroxisome proliferator-activated receptor α-knockout mouse.
    Article Snippet: .. To assess PDH activity state, protein expression of PDK1, 2, and 4 were measured (Primary anti-bodies for PDK1: #3820 Cell signalling Technology, Boston, MA, USA, PDK2: AP7039b Abgent, Maidenhead, UK, PDK4: a kind gift from Prof Mary Sugden, University of London). .. Protein levels of MCAD and UCP3 were measured to assess protein expression of genes under the transcriptional control of PPARα (Primary anti-bodies for MCAD: sc-49047 Santa Cruz, Texas, USA; UCP3: ab3477 Abcam, Cambridge, UK).

    Expressing:

    Article Title: The age-dependent development of abnormal cardiac metabolism in the peroxisome proliferator-activated receptor α-knockout mouse.
    Article Snippet: .. To assess PDH activity state, protein expression of PDK1, 2, and 4 were measured (Primary anti-bodies for PDK1: #3820 Cell signalling Technology, Boston, MA, USA, PDK2: AP7039b Abgent, Maidenhead, UK, PDK4: a kind gift from Prof Mary Sugden, University of London). .. Protein levels of MCAD and UCP3 were measured to assess protein expression of genes under the transcriptional control of PPARα (Primary anti-bodies for MCAD: sc-49047 Santa Cruz, Texas, USA; UCP3: ab3477 Abcam, Cambridge, UK).

    Electrophoresis:

    Article Title: The H3K27 acetyltransferase p300 is dispensable for thermogenic adipose tissue formation and function
    Article Snippet: Protein concentration was determined by the BCA assay (ThermoScientific) using bovine serum albumin as standards. .. Following electrophoresis, samples were wet-transferred onto PVDF membranes (pore size: 0.2 μm) and probed overnight at 4°C for UCP-1 (ab10983, Abcam), mitochondrial respiratory chain subunits (total OXPHOS; ab110413, Abcam), and for the following using primary anti-bodies from Cell Signalling Technologies: green fluorescent protein (GFP; 4B10), acetylated H3K27 (Lys27), H3 (D1H2), and ß-Tubulin (D3U1W). .. Following incubation in appropriate secondary anti-bodies (CST), immunoblots were visualized on film using enhanced chemiluminescent substrates.

    Pore Size:

    Article Title: The H3K27 acetyltransferase p300 is dispensable for thermogenic adipose tissue formation and function
    Article Snippet: Protein concentration was determined by the BCA assay (ThermoScientific) using bovine serum albumin as standards. .. Following electrophoresis, samples were wet-transferred onto PVDF membranes (pore size: 0.2 μm) and probed overnight at 4°C for UCP-1 (ab10983, Abcam), mitochondrial respiratory chain subunits (total OXPHOS; ab110413, Abcam), and for the following using primary anti-bodies from Cell Signalling Technologies: green fluorescent protein (GFP; 4B10), acetylated H3K27 (Lys27), H3 (D1H2), and ß-Tubulin (D3U1W). .. Following incubation in appropriate secondary anti-bodies (CST), immunoblots were visualized on film using enhanced chemiluminescent substrates.



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